Methodologies

Peptide Literature

References

Product Use and Liability

Induction of outward current by orexin-B in mouse peritoneal macrophages

Title

Induction of outward current by orexin-B in mouse peritoneal macrophages.

Author

Ichinose M; Asai M; Sawada M; Sasaki K; Oomura Y

Source

FEBS Lett, 440(1-2):51-4 1998 Nov 27

Abstract

To define effects of novel feeding regulating peptides, orexins, in immunocompetent cells, ion channel activity in mouse peritoneal macrophages was analyzed by the perforated patch-clamp method. Orexin-B (OX-B) induced an outward current at smaller holding potentials than K+ equilibrium potentials. Reversal potentials of OX-B induced current were dependent on external K+ concentrations but not on external Cl- concentration. Orexin-A is less effective than OX-B. Quinine blocked the outward current and tetraethylammonium partially suppressed the current. These results suggest that OX-B can modulate macrophage functions through the activation of Ca2+-dependent K2+ channels.

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